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Combining Kunkel mutagenesis and phage-display to create bivalent affinity reagents

Details

Project TitleCombining Kunkel mutagenesis and phage-display to create bivalent affinity reagents
Track CodeUIC-2014-141
Short Description

This invention is a highly efficient bivalent reagent creation method that is less labor intensive and time intensive than conventional epitope binning practices.


Abstract

Megaprimer Shuffling for Tandem Affinity Reagents (MegaSTAR) is a UIC technology that converts the output of affinity selection to a bivalent display format. Bivalent reagents have many applications however due to their labor intensive creation, they have been avoided as a viable solution. UIC researchers have invented a method using phage-display and Kunkel mutagenesis to create bivalent affinity reagents. These molecules bind 2 distinct areas(epitopes) on the same target protein. This novel method allows for the rapid and efficient generation of these reagents in a high throughput manner to theoretically any target. Epitope binning consists of examining pairs of binders (in soluble protein form) to determine if they compete for the same epitope. This invention allows one to examine many different combinations of affinity reagents without the need for pair-wise analysis. This MegaSTAR technology relies on synthesizing a "bivalent library" by first generating a pool of megaprimers from selection output, and annealing them randomly to a bivalent vector. This new "bivalent library" is then used for further affinity selection to identify tandem reagents with the highest affinity. This greatly reduces the time and cost of identifying pairs of reagents that bind to different epitopes on the same target protein


Benefits:

  • Application in diagnostics as well as reagents for cell studies.
  • Decreased labor time
  • Lower cost, higher specificity

For more information, contact Chuck Na.

 
Tagsaffinity reagents, bivalent reagents, library
 
Posted DateMar 17, 2016 10:48 AM

Researcher

Name
Renhua Huang
Kevin Gorman
Brian Kay

Manager

Name
Chuck Na
Sandra Thompson