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LoxP xPress: a novel system for serial cloning

Details

Project TitleLoxP xPress: a novel system for serial cloning
Track CodeINV 30061
Short DescriptionA series of novel loxP spacer sites with the potential to be developed into a novel platform for powerfully expanding the potential of the routinely used Cre-LoxP system.
AbstractNone
 
Tagsbioinformatics, biomedical, biotechnology, genetics, genomics, health & life sciences, r&d discovery
 
Posted DateJan 27, 2010 9:21 AM

Description

This collection of novel loxP spacer sites has the potential to be developed into a novel system for powerfully expanding the potential of the routinely used Cre-LoxP system.

Potential Applications

· Simple and rapid cloning system

· Suitable for engineering large DNA constructs in vivo

· Applicable to all prokaryote and eukaryote organisms where Cre-LoxP is deployed

· Applicable to mutant LoxP sites mouse transgenics, where the LoxP system is used extensively. These mutant sites could in principle be used to make serial insertions into mouse genome.

· Synthetic biology applications

State of Development

Proof of concept developed. Not yet demonstrated in murine system. Further development needed to develop commercial system.

Background

Dr Rob Holt developed a system useful for mediating direct serial insertions into a given target construct, chromosome or genome. A set of LoxP sequences was identified as having restricted promiscuity, and therefore potential utility for mediating serial, non-interacting recombinations (aka multisite gateway).

Published: A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination.  P.I. Missirlis, D.E. Smailus and R. A Holt.  04 April 2006 BMC Genomics 2006, 7:73 

Advantages

· Multiple Self Recombining sites

· Multiple Restricted Recombination sites

· Compatible with standard Cre-LoxP system

· Make serial insertions into mouse genomes

· Simple and rapid to use

Opportunity

BCCA is seeking licensing agreement with a commercial partner