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Anti-Map2K5-FN3 affinity reagent

Details

Project TitleAnti-Map2K5-FN3 affinity reagent
Track CodeUIC-2015-149
Short Description

The reagent is a variant of a fibronectin type III domain (FN3) scaffold isolated from a phage-display library after affinity selection. It is renewable, easily produced via expression in E. coli, and has affinity in the low nanomolar (1-50 nM) range. The reagent has been tested in ELISA and pull-down experiments.

Abstract

Traditionally, affinity reagents such as antibodies are generated using animals. Unfortunately this process is time-consuming and costly, sometimes taking months to isolate binding reagents. In addition, the animals used are not a renewable source of material and are expensive to maintain. In fact, many antibodies generated via animal methods are promiscuous, binding to more than one target.

A different strategy is to isolate binding reagents is to use phage-display. In this case, the process is performed entirely in vitro, and the reagents isolated are recombinant (thus renewable). Furthermore, the process itself is much faster than traditional methods, taking only a few weeks. Researchers at UIC have streamlined this process, using phage-display to isolate binding reagents and subsequently using affinity maturation to increase affinity of the reagent into the low nanomolar range. These reagents have been shown to be specific for their cognate target, and some have even been used in pull-down experiments.

Applications

  • ELISA
  • Pulldown

Benefits

  • Renewable
  • Recombinant
  • Easy-to-produce
  • Reduces time and cost

For more information, contact Nelson Grihalde

 
Tagsantibodies, Research Tools & Reagents
 
Posted DateMar 14, 2016 11:19 AM

Researcher

Name
Michael Kierny
Brian Kay
Christopher Vinci

Manager

Name
Chuck Na
Sandra Thompson