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Anti-LYN-FN3 affinity reagent

Details

Project TitleAnti-LYN-FN3 affinity reagent
Track CodeUIC-2015-150
Short Description

The reagent is a variant of a fibronectin type III domain (FN3) scaffold isolated from a phage-display library after affinity selection. It is renewable, easily produced via expression in E. coli. The reagent has been tested in ELISA and pull-down experiments.

Abstract

Traditionally, affinity reagents such as antibodies are generated using animals. Unfortunately this process is time-consuming and costly, sometimes taking months to isolate binding reagents. In addition, the animals used are not a renewable source of material and are expensive to maintain. In fact, many antibodies generated via animal methods are promiscuous, binding to more than one target.

A different strategy is to isolate binding reagents is to use phage-display. In this case, the process is performed entirely in vitro, and the reagents isolated are recombinant (thus renewable). Furthermore, the process itself is much faster than traditional methods, taking only a few weeks. Researchers at UIC have streamlined this process, using phage-display to isolate binding reagents and subsequently using affinity maturation to increase affinity of the reagent into the low nanomolar range. The reagent has been successfully used in ELISA and pulldown.

Applications

  • ELISA
  • Pull-down

Benefits

  • Renewable
  • Recombinant
  • Easy-to-produce
  • Reduces time and cost
For more information, contact Nelson Grihalde
 
Tagsantibodies, Research Tools & Reagents
 
Posted DateMar 14, 2016 11:31 AM

Researcher

Name
Brian Kay
Renhua Huang

Manager

Name
Chuck Na
Sandra Thompson