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Anti-USP11-FN3 affinity reagent

Details

Project TitleAnti-USP11-FN3 affinity reagent
Track CodeUIC-2015-147
Short Description

The reagent is a variant of a fibronectin type III domain (FN3) scaffold isolated from a phage-display library after affinity selection. It is renewable, easily produced via expression in E. coli. The reagent has been successfully used in ELISA and pull-down.

Abstract

Traditionally, affinity reagents such as antibodies are generated using animals. Unfortunately this process is time-consuming and costly, sometimes taking months to isolate binding reagents. In addition, the animals used are not a renewable source of material and are expensive to maintain. In fact, many antibodies generated via animal methods are promiscuous, binding to more than one target.

A different strategy is to isolate binding reagents is to use phage-display. In this case, the process is performed entirely in vitro, and the reagents isolated are recombinant (thus renewable). Furthermore, the process itself is much faster than traditional methods, taking only a few weeks. Researchers at UIC have streamlined this process, using phage-display to isolate binding reagents and subsequently using affinity maturation to increase affinity of the reagent. The reagent has been successfully used in ELISA and pull-down.

Applications

  • ELISA
  • Pull-down

Benefits

  • Renewable
  • Recombinant
  • Easy-to-produce
  • Reduces time and cost
For more information, contact Nelson Grihalde
 
Tagsantibodies, Research Tools & Reagents
 
Posted DateMar 14, 2016 12:58 PM

Researcher

Name
Kevin Gorman
Michael Kierny
Brian Kay
Renhua Huang
Christopher Vinci

Manager

Name
Chuck Na
Sandra Thompson