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Automated Rapid Reaction Screening by DESI-MS

Details

Project TitleAutomated Rapid Reaction Screening by DESI-MS
Track Code2017-COOK-67863
Websitehttp://otc-prf.org/
Short Description

R. Graham Cooks

Purdue Chemistry

Aston Labs

Abstract
 
TagsChemistry and Chemical Analysis, DESI, ionization, mass spectrometry
 
Posted DateSep 14, 2017 9:26 AM

Researcher

Name
Robert Graham Cooks
Christina R Ferreira
Michael Wleklinski

Automated Rapid Reaction Screening by DESI-MS

Background

Combinatorial chemistry involves chemical synthetic methods that make it possible to prepare a large number of compounds in a single process.  There are many limitations with current processes including the use of separate systems for reaction synthesis and reaction screening. Typically, a robotic instrument combines reagents and conducts reactions, which can vary from minutes to hours, to even days. Once completed, the transfer to a screening instrument, such as a mass spectrometer, takes place after the manual sample of each reaction product and the manual creation of an array of reaction products on a substrate for screening. The screening of each reaction product is time consuming. Given the reaction product is manually prepared, numerous errors, such as contaminated or mixed-up samples, leads to improper data.  Repeating the entire process is the only solution.

Technology Summary

Researchers at Purdue University have developed systems and methods for conducting reactions and screening for reaction products. This method is based on desorption electrospray ionization (DESI) of the reaction mixture to produce microdroplets containing the reagents. Within the droplets, the reagents can react at accelerated rates. The droplets are directed towards a mass spectrometer (MS) and their contents are monitored online using MS. This method offers accelerated screened reactions, with analysis performed online and in concert with reaction, and the speed of DESI analysis allows for the analysis in a single experiment of a large numbers of different reactions mixtures.

Advantages

  • Rapid
  • Automatic
  • Uses existing equipment

Potential Applications

  • Chemical analysis
  • Microfluidics

Stage of Development

Concept validated through theory, simulation, or models

Web Links

For additional information, please contact otcip@prf.org.

Intellectual Property

Patent Number Issue Date Type Country of Filing
None None Provisional United States