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Monoclonal Antibody Panel Against Glycoproteins G1 and G2 of Tomato Spotted Wilt Virus (TSWV, Tospovirus) (8 lines)

Details

Project TitleMonoclonal Antibody Panel Against Glycoproteins G1 and G2 of Tomato Spotted Wilt Virus (TSWV, Tospovirus) (8 lines)
Track Code2426
Short Description

Applications

Suitable for DAS-ELISA, PAS-ELISA and DIBA applications.Tomato spotted wilt tospovirus (TSWV) is the type member of the plant-infecting viruses of the genus Tospovirus in the family Bunyaviridae. TSWV is transmitted by thrips (Thysanoptera: Thripidae) to over 650 different plant species in temperate and tropical regions. Once the plants infected with the virus, they result in spotting, wilting, reduced vegetative output and death of the plant. Some of the crops known to be affected by the viruses include, peanuts, watermelons, tomatoes, zucchinis and others.

Abstract

Reagents Description

 

Clone Name

Antigen

Immunogen

MW (KDa)

Reactivity/Recognition

Tested Applications

G1-5D5.G5

G1 glycoprotein of TSWV

Glycoprotein

95

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF

5F11

G2 Glycoprotein of TSWV

Glycoprotein

58

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF

6G8

G2 Glycoprotein of TSWV

Glycoprotein

58

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF

G2.1D6.H11

G2 Glycoprotein of TSWV

Glycoprotein

58

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF

G2

G2 Glycoprotein of TSWV

Glycoprotein

58

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF, IB

G2-1G6.A8

G2 Glycoprotein of TSWV

Glycoprotein

58

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF, IB

G2-6D1.KC6

G2 Glycoprotein of TSWV

Glycoprotein

58

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF

HL-1.G2

G2 Glycoprotein of TSWV

Glycoprotein

58

TSWV, possibly other Tospoviruses or Bunyaviridae

ELISA, WB, IF

Species Immunized:  Mouse

Purification Method:  Unpurified. Used as supernatant

Antibody storage temperature: -80oC

References

 

Sherwood, JL, Sanborn, MR, Keyser, GC; Myers, LD. Use of Monoclonal Antibodies in Detection of Tomato Spotted Wilt Virus, Phytopathology, 79(1), 61-64 (1989).

Bandla, MD, Daphne, MW; Chenault, K.D.; Ullman, DE; German, TL; Sherwood, JL: Use of Monoclonal Antibody to the Nonstructural Protein Encoded by the Small RNA of Tomato Spotted Wilt Tospovirus to Identify Viruliferous Thirps: Phytopathology, 84(12), 1427-1431 (1994).

Naidu, RA; Sherwood, JL; Deom, CM. Characterization of a vector-non-transmissible isolate of Tomato spotted wilt virus. Plant Pathol, 57, 190-200 (2008).

Richmond, KE, Chenault, K; Sherwood, JL; Geramn, TL: Characterization of the Nucleic Acid Binding Properties of Tomato Spotted Wilt Virus Nucleocapsid Protein, Virology 248, 6-11 (1998).

Adkins, S, Choi, T-J,Israel, BA, Bandla, MD, Richmond, KE, Schultz, KT, Sherwood, JL, German, TL. Baculovirus Expression and Processing of Tomato Spotted Wilt Topovirus Glycoproteins. Phytopathology 86(8), 849-855 (1996).

 
Tagsglycoproteins, Tomato Spotted Wilt Virus, TSWV, antibody
 
Posted DateNov 14, 2017 1:27 PM

People

Name
Gennaro Gama

Files

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TSWV Glycoproteins.docx None Download