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Bacterial Hexose Formation: Sugar conversions, one-carbon fixation and the accumulation of sugar-derived products in Escherichia coli

Details

Project TitleBacterial Hexose Formation: Sugar conversions, one-carbon fixation and the accumulation of sugar-derived products in Escherichia coli
Track Code2028
Short Description

Genetically engineered E. coli which converts pentoses into hexoses, via capture of one-carbon compounds (e.g., CO2, CH3OH, etc.). The microorganism accumulates that hexose and makes it available to metabolism into products of interest, such as commodity chemicals, biofuels, and rare/unnatural sugars.

 

Glucose is the primary currency of energy for much of life. Plant, bacterial and mammalian cells have evolved highly efficient biochemical pathways to oxidize glucose, not only to generate energy but also to synthesize precursor molecules used as the building blocks of cellular materials. Many cells have also devised mechanisms to store glucose in different forms: plants generate starch and cellulose, while many eukaryotic cells accumulate glycogen. In addition to precursor molecules and storage products, structurally diverse compounds are derived from glucose and other monosaccharides; for example, glycosylation of small molecules and proteins provides unique cellular functionalities. Promoting hexose accumulation beyond natural levels constitutes an opportunity for better understanding of monosaccharide interconversions, and as a platform for the generation – in much higher yields -  of important products derived from these intermediate hexoses. The accumulation of hexoses directly from pentoses might advance the use of lignocellulosic hydrolysates with organisms such as Saccharomyces cerevisiae which metabolize D-glucose readily but are natively unable to consume pentoses

Target products and synonyms: Hexoses, C6 sugars, aldohesoxes. Talose, ribose, lyxose

 

Target identifiers: Hexoses, aldohexoses, glycosylated compounds

Abstract

Genetically engineered E. coli which converts pentoses into hexoses, via capture of one-carbon compounds (e.g., CO2, CH3OH, etc.). The microorganism accumulates that hexose and makes it available to metabolism into products of interest, such as commodity chemicals, biofuels, and rare/unnatural sugars.

 

Glucose is the primary currency of energy for much of life. Plant, bacterial and mammalian cells have evolved highly efficient biochemical pathways to oxidize glucose, not only to generate energy but also to synthesize precursor molecules used as the building blocks of cellular materials. Many cells have also devised mechanisms to store glucose in different forms: plants generate starch and cellulose, while many eukaryotic cells accumulate glycogen. In addition to precursor molecules and storage products, structurally diverse compounds are derived from glucose and other monosaccharides; for example, glycosylation of small molecules and proteins provides unique cellular functionalities. Promoting hexose accumulation beyond natural levels constitutes an opportunity for better understanding of monosaccharide interconversions, and as a platform for the generation – in much higher yields -  of important products derived from these intermediate hexoses. The accumulation of hexoses directly from pentoses might advance the use of lignocellulosic hydrolysates with organisms such as Saccharomyces cerevisiae which metabolize D-glucose readily but are natively unable to consume pentoses.

 

Technology Description

 

The invention includes bacterial strains which accumulate a six carbon sugar ("hexose") such as glucose and mannose as a final product in a medium when supplied with five carbon sugars ("pentose") such as xylose or arabinose or even when supplied with glycerol.  Since the cells can be rendered unable to metabolize certain sugars but otherwise still grow and function biologically, this invention affords the opportunity to direct these hexoses into valuable products such as the so-called "rare" (unnatural) sugars (e.g., talose, ribose, lyxose, all of relevance in the development of therapeutics) and to a wide variety of glycosylated (glucuro-, gluco-, galacto-, manno-derivatized) compounds such as cyanidin 3-glucoside.  Furthermore, this invention serves as a means of building onto a carbon backbone from, for example, 5-carbon to 6-carbon length. The peculiar design of these strains make them attractive to undergo further engineering targeting the production of a large scope of industrial chemicals, such as, acids, diacids, furans, diols and others.

 

Technology successfully implemented under both continuous process and batch process with mass conversion yields that have been demonstrated to exceed 30% (>0.30 g/g).

  • ~References and Intellectual Property
Patent pending
 
TagsHexoses, C6 sugars, aldohesoxes, talose, ribose, lyxose
 
Posted DateNov 14, 2017 12:01 PM

People

Name
Gennaro Gama

Files

File Name Description
2028 Eiteman GG.doc None Download